Recent Publications

Beckwith, J. Clever Mutant Isolation: Defining the Components of Cell Biological Processes. In: Stanley Maloy and Kelly Hughes, editors. Brenner's Encyclopedia of Genetics 2nd edition, Vol 2. San Diego: Academic Press; 2013. p. 33-37.

Beckwith, J. The Sec-dependent pathway, Res. Microbiol. 164:497-504 (2013).

Beckwith, J. 50 years Fused to Lac. Ann. Rev. Microbiol. (Intro Chapter). (in press, 2013).

Chng, S.-S., Xue, M., Garner, R.A., Kadokura, H., Boyd, D., Beckwith, J. and Kahne, D. Disulfide Rearrangement Triggered by Translocon Assembly Controls Lipopolysaccharide Export. Science 337:1665-1668 (2012).

Chng, S.-S., Dutton, R.J., Denoncin, K., Vertommen, D., Collet, I.-F., Kadokura, H. and Beckwith, J. Overexpression of the rhodanese PspE, a single cysteine-containing protein, restores disulfide bond formation to an Escherichia coli strain lacking DsbA. Mol. Microbiol. 85:996-1006 (2012).

Feeney, M., Ke, N., and Beckwith, J. Mutations at several loci cause increased expression of ribonucleotide reductase in Escherichia coli. J. Bacteriol. 194:1515-1522 (2012).

Cho, S.-H., Parsonage, D., Dutton, R., Thurston, C., Poole, L., Collet, J.-F., and Beckwith, J.. A new family of membrane electron transporters and its substrates, including the first cell-envelope peroxiredoxin, reveal a broadened reductive capacity of the oxidative bacterial cell envelope. MBio 3:1-11 (2012).

Robichon, C., Karimova, G., Beckwith, J., and Ladant, D. Role of leucine zipper in the association of the Escherichia coli cell division proteins FtsL and FtsB, J. Bacteriol. 193:4988-4922 (2011).

Feeney, M., Gon, S., Veeravalli, K., Faulkner, M.J., Georgiou, G. and Beckwith, J. Repurposing lipoic acid changes electron flow in two important metabolic pathways of Escherichia coli. Proc. Natl. Acad. Sci. 108:7991-7996 (2011).

Beckwith, J. The Operon as Paradigm: Normal Science and the Beginning of Biological Complexity. J. Mol. Biol. 409: 7-13 (2011).

Wang, X., Dutton, R., Beckwith, J., and Boyd, D. Membrane topology and mutational analysis of Mycobacterium tuberculosis VKOR, a protein involved in disulfide bond formation and a homologue of human vitamin K epoxide reductase. Antioxidants and Redox Signalling. 14:1413-1420 (2011).

Veeravalli, K., Boyd, D., Iverson, B.L., Beckwith, J., and Georgiou, G. Evolution of de novo biosynthetic pathways for γ-glutamyl cysteine and unnatural amino-thiol analogues. Nat. Chem.Biol.7:101-5. (2011).

Dutton, R.J., Wayman, A., Wei, J.-R., Rubin, E.J., Beckwith, J., and Boyd, D. Inhibition of bacterial disulfide bond formation by the anti-coagulant warfarin. Proc. Natl. Acad. Sci.(in press).

Kadokura, H., and Beckwith, J. Detecting folding intermediates of a protein as it passes through the bacterial translocon. Cell 138:1164-1173 (2009).

Dutton, R.J., Boyd, D., Berkmen. M., and Beckwith, J. Bacterial species exhibit diversity in their mechanisms and capacity for protein disulfide bond formation. Proc. Natl. Acad. Sci., U.S.A. 105:11933-11938 (2008).

Faulkner, M.J., Veeravalli, K., Gon, S., Georgiou, G., and Beckwith, J. Functional plasticity of a peroxidase allows evolution of diverse disulfide-reducing pathways. Proc. Natl. Acad. Sci. U.S.A. 105:6735-40 (2008).

Beckwith, J. What Lies Beyond Uranus? Preconceptions, Ignorance, Serendipity and Suppressors in the Search for Biology's Secrets. Genetics 176:733-740 (2007).

Cho, S.-H., Porat, A., Ye, J., and Beckwith, J. Redox-active cysteines of a membrane electron transporter DsbD show dual compartment accessibility. EMBO J. 8:3509-3520 (2007).


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George Georgiou, Department of Chemistry and Chemical Engineering, University of Texas, Austin TX.
With Dr. Georgiou we are studying the details of the system for isomerizing disulfide bonds in E. coli for the purpose of engineering strains that are more efficient at expressing eukaryotic proteins with multiple disulfide bonds.

Alain Chaffotte, Repliement et Modélisation de Protéines, Dépt. de Biologie Structurale et Chimie, INSTITUT PASTEUR, Paris - France.

Maria Luisa Tasayco, Department of Chemistry, The City College of New York, New York, NY.

Elliot Crooke, Georgetown University School of Medicine, Washington, DC.

Daniel Ladant, Biochimie des Interactions macromoléculaires, Dépt. de Biologie Structurale et Chimie, INSTITUT PASTEUR, Paris - France.

Tom Rapoport, Department of Cell Biology, Harvard Medical School, Boston, MA.

Arne Holmgren, Medical Nobel Institute for Biochemistry, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.

Kirsten Skarstad, Department of Cell Biology, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo, Norway.